Approaches in vivo and in vitro for solving the vascularization issue of brain organoids

Tianyi Li

doi:10.54097/vhxbb234

Authors

Tianyi Li

DOI:

https://doi.org/10.54097/vhxbb234

Keywords:

Organoids, Vascularization, Xenotransplantation, Active blood flow, Stroke recovery.

Abstract

Organoids are three-dimensional tissue cultures generated using human pluripotent stem cells. It shows great potential in modeling human disorders, organogenesis, and developmental disorders. However, they have limitations in replicating the complexity of the human brain and lack a complete vasculature to support long-term cultivation and endogenous microenvironment simulation. Researchers are exploring strategies to promote vascularization in brain organoids, including in vitro co-culture methods and xenotransplantation into highly vascularized regions of animal hosts. The presence of a physiologically perfused vasculature in organoid models can prevent tissue necrosis, provide essential nutrients, and enable accurate modeling of interactions with non-neuronal cell types. In vivo, xenotransplantation shows advantages over in vitro approaches, such as active blood flow, and demonstrates potential benefits for aiding recovery from stroke by repairing damaged tissue structures and improving sensory-motor deficits.

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References

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