Research on the Application of Gene Coding Fluorescent Probes
DOI:
https://doi.org/10.54097/27be3a29Keywords:
Genetically encoded fluorescent probe, metal ion detection, neurotransmitter imaging, cellular metabolism.Abstract
Genetically encoded fluorescent probes, renowned for their biocompatibility and suitability for live-cell detection, have emerged as a pivotal tool in the field of molecular and ionic detection. These probes are characterized by their high specificity, exceptional editability, and rapid imaging capabilities, making them indispensable in many biological assays. This review aims to elucidate the mechanisms and applications of gene-encoded fluorescent probes in metal ion detection, neurotransmitter imaging, and cell metabolism detection. Specifically, probes constructed through gene programming can cause changes in fluorescence intensity by binding specifically to target substances, thereby achieving accurate concentration detection and real-time imaging of target substances. The biological compatibility of gene-encoded fluorescent probes is good, and they can be used in living cells, so they also have important value in the detection of physiological and pathological indicators. This review will also delineate the comparative advantages of these probes over traditional detection methods and offer insights into the prospective trajectory of this burgeoning technology.
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