Chitinase-binding gene BvCHiB enhances resistance of Arabidopsis to verticillium wilt through JA and SA pathways

Yujing Liu

doi:10.54097/40b93n71

Authors

Yujing Liu

DOI:

https://doi.org/10.54097/40b93n71

Keywords:

B. velezensis, upland cotton, disease resistance, Verticillium dahliae.

Abstract

To date, no ideal effective method for controlling Verticillium wilt in upland cotton (Gossypium hirsutum) has been defined. There are few studies on BvCHiB gene from B. velezensis to improve plant disease resistance mechanism. The purpose of this study was to determine the effects and mechanism through which BvCHiB enhances plant disease resistance, and increases resistance to Verticillium wilt. The BvCHiB gene was cloned from an endophytic bacterium (B. velezensis) isolated from roots of the upland cotton cultivar Zhongzhimian 2. Transgenic Arabidopsis plants overexpressing BvCHiB showed significantly improved resistance to Verticillium wilt. The accumulation of resistance related substances such as lignin, PAL, POD, SOD, CAT and MDA increased significantly 48h after inoculation with verticillium wilt. These immune resistant substances can effectively resist the infection of verticillium wilt. In addition, the expression of related resistance genes in JA and SA pathway was significantly higher than that in control. Therefore, BvCHiB may enhance disease resistance of plants mainly through JA and SA signaling pathway. BvCHiB may be used as a resistance gene to improve resistance to V. dahliae in upland cotton.

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