Overexpression of Splicing Variants VvPMA1β from Vitis Vinifera L. Improve Salt Tolerance in Arabidopsis Thaliana

Lanxin Li; Fengjie Wang; Zhen Yang; Pengrui Wang; Ning Han

doi:10.54097/nq3hdj87

Authors

Lanxin Li
Fengjie Wang
Zhen Yang
Pengrui Wang
Ning Han

DOI:

https://doi.org/10.54097/nq3hdj87

Keywords:

Grape, VvPMA1, overexpression, salt stress

Abstract

Plasma membrane H⁺-ATPase (PMA) plays a very important role in regulating plant response to salt stress and decreasing Na⁺/K⁺ in cytosol. We found earlier that two splicing variants, VvPMA1α and VvPMA1β, were produced from VvPMA1 gene in grape root plasma membrane under salt stress. But the salt-resistant activity of VvPMA1 splicing variants was unknown. Here, the two variants were overexpressed in Arabidopsis thaliana and then salt tolerance of transgenic plants was measured. The results showed that the seed germination rate, root length and reproductive growth of the VvPMA1α and VvPMA1β overexpressing plants were significantly better than those of the wild type under salt stress. Furthermore, compare with VvPMA1α overexpressing plants, VvPMA1β overexpressing plants displayed superior performance. And then, A. thaliana with VvPMA1β overexpression was selected to determine its salt tolerance activity. The results showed that the growth of VvPMA1β transgenic plants under salt stress was better than that of wild type, and the content of proline was significantly higher than that of wild type, while MDA and Na⁺/K⁺ were significantly lower than that of wild type. Therefore, the VvPMA1β variant produced from VvPMA1 gene alternative splicing was helpful to salt resistance of plants under salt stress.

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