Cold Resistance in the Callus of Vitis Vinifera ‘Zaoxia’ Was Enhanced by Overexpressing the VyDHN1 Gene

Muqing Liu; Heqiang Chang; Lingmin Dai; Guomin Han

doi:10.54097/xm3m5y47

Authors

Muqing Liu
Heqiang Chang
Lingmin Dai
Guomin Han

DOI:

https://doi.org/10.54097/xm3m5y47

Keywords:

VyDHN1, callus, genetic transformation, cold resistance

Abstract

The cold resistance of grapevine (vitis vinifera L.) which is one of the most important economic fruit trees in the world is low. The grown distribution of grapevine was restricted by climate and other factors. Dehydrins (DHN) are a class of proteins that play an important role in plants’ defense against stress. The VyDHN1 was cloned from V. yeshanensis which is highly resistant to cold stress, but the function and related mechanism of this gene in response to cold stress in grapevine has not been elucidated yet. In this study, the genetic transformation system was successfully established using callus induced by exocarp tissue of table grape varieties (V. vinifera ‘Zaoxia’ and ‘87-1’) by agrobacterium-mediated method. The VyDHN1 gene was stably overexpressed into the callus and the physiological indicators of the transgenic callus were detected under cold stress. The study revealed that VyDHN1 overexpression can maintain the cell membrane stability of the transgenic callus of Zaoxia during cold stress which could improve the cold resistance of grapevine.

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